Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations.
نویسندگان
چکیده
منابع مشابه
Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations.
The enzyme, 5'-nucleotidase (5'N) (E.C.-3.1.3.5) is present in lymphocytes isolated from the blood of normal subjects. This activity is markedly decreased or not detectable in the cells from three-quarters of patients with chronic lymphocytic leukemia (CLL), while supranormal levels are found in less than 10% of the cases. To determine whether the decreased 5'N value in CLL represents a lower a...
متن کاملFunctional characterization of ecto-5'-nucleotidase-positive and -negative human T lymphocytes.
Functional studies were performed on human peripheral blood T lymphocytes stained with goat anti-5'-nucleotidase antibodies and separated into ecto-5'-nucleotidase (ecto-5'-NT)-positive and -negative populations using the FACSTAR fluorescence-activated cell sorter. On the average, ecto-5'-NT+ T cells contained 34 +/- 13% CD4+ and 55 +/- 15% CD8+ cells, whereas ecto-5'-NT-T cells contained 65 +/...
متن کاملFunction of 5’-Nucleotidase in the Uptake of Adenosine from AMP by Human Lymphocytes*
The function of 5’-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5’N+) with that in cells deficient in the activity (5’N-). The rate of adenosine and P, uptake from AMP was 3.9-fold greater in the 5’N+ then in the 5’N lymphoc$tes. There was no difference in transport between these cells when incubated...
متن کاملFunction of 5'-nucleotidase in the uptake of adenosine from AMP by human lymphocytes.
The function of 5'-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5'N+) with that in cells deficient in the activity (5'N-). The rate of adenosine and Pi uptake from AMP was 3.9-fold greater in the 5'N+ then in the 5'N- lymphocytes. There was no difference in transport between these cells when incubate...
متن کاملIdentification of thymidine nucleotidase and deoxyribonucleotidase activities among normal isozymes of 5'-nucleotidase in human erythrocytes.
The persistence of normal thymidine nucleotidase (ThyNase) activity in subjects with pyrimidine nucleotidase (PyrNase) deficiency suggested the possible existence of separate isozymes in normal human erythrocytes. This hypothesis was confirmed by studies of PyrNase-deficient individuals from five unrelated families. Erythrocytes deficient in PyrNase retained normal activity of an enzyme system ...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1975
ISSN: 0021-9738
DOI: 10.1172/jci108209